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PTEN was downregulated in THCA cells. A : GEPIA database was used to analyze the expression of PTEN in THCA tumor tissues; Red box plots indicated the Tumor group (n = 512); Gray box plots represented the Normal group (n = 337); Log2(TPM + 1) served as the unit of gene expression in the Y axis; B : ENCORI database was used to analyze the PTEN expression in THCA tumor tissues; C – D : RT-qPCR and Western blot were employed to confirm mRNA and protein levels of PTEN in normal thyroid <t>follicular</t> <t>epithelial</t> cells Nthy-ori 3–1 and THCA cells B-CPAP, FTC-133, and TPC-1, respectively. Data were expressed as mean ± SD, and cellular experiments were repeated three times independently. One-way analysis of variance was utilized for comparison among multiple groups, * P < 0.05, *** P < 0.001
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Fig. 1 PTEN was downregulated in THCA cells. A: GEPIA database was used to analyze the expression of PTEN in THCA tumor tissues; Red box plots indicated the Tumor group (n = 512); Gray box plots represented the Normal group (n = 337); Log2(TPM + 1) served as the unit of gene expression in the Y axis; B: ENCORI database was used to analyze the PTEN expression in THCA tumor tissues; C–D: RT-qPCR and West- ern blot were employed to confirm mRNA and protein levels of PTEN in normal thyroid <t>follicular</t> <t>epithelial</t> cells Nthy-ori 3–1 and THCA cells B-CPAP, FTC-133, and TPC-1, respectively. Data were expressed as mean ± SD, and cellular experiments were repeated three times indepen- dently. One-way analysis of variance was utilized for comparison among multiple groups, * P < 0.05, *** P < 0.001
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PTEN was downregulated in THCA cells. A : GEPIA database was used to analyze the expression of PTEN in THCA tumor tissues; Red box plots indicated the Tumor group (n = 512); Gray box plots represented the Normal group (n = 337); Log2(TPM + 1) served as the unit of gene expression in the Y axis; B : ENCORI database was used to analyze the PTEN expression in THCA tumor tissues; C – D : RT-qPCR and Western blot were employed to confirm mRNA and protein levels of PTEN in normal thyroid follicular epithelial cells Nthy-ori 3–1 and THCA cells B-CPAP, FTC-133, and TPC-1, respectively. Data were expressed as mean ± SD, and cellular experiments were repeated three times independently. One-way analysis of variance was utilized for comparison among multiple groups, * P < 0.05, *** P < 0.001

Journal: Discover Oncology

Article Title: PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway

doi: 10.1007/s12672-024-01596-8

Figure Lengend Snippet: PTEN was downregulated in THCA cells. A : GEPIA database was used to analyze the expression of PTEN in THCA tumor tissues; Red box plots indicated the Tumor group (n = 512); Gray box plots represented the Normal group (n = 337); Log2(TPM + 1) served as the unit of gene expression in the Y axis; B : ENCORI database was used to analyze the PTEN expression in THCA tumor tissues; C – D : RT-qPCR and Western blot were employed to confirm mRNA and protein levels of PTEN in normal thyroid follicular epithelial cells Nthy-ori 3–1 and THCA cells B-CPAP, FTC-133, and TPC-1, respectively. Data were expressed as mean ± SD, and cellular experiments were repeated three times independently. One-way analysis of variance was utilized for comparison among multiple groups, * P < 0.05, *** P < 0.001

Article Snippet: Normal human thyroid follicular epithelial cells Nthy-ori 3–1 (CL-0817) and human THCA cell lines B-CPAP (CL-0575), FTC-133 (CL-0644) and TPC-1 (CL-0643) were provided by Procell (Wuhan, Hubei, China).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Western Blot, Comparison

Fig. 1 PTEN was downregulated in THCA cells. A: GEPIA database was used to analyze the expression of PTEN in THCA tumor tissues; Red box plots indicated the Tumor group (n = 512); Gray box plots represented the Normal group (n = 337); Log2(TPM + 1) served as the unit of gene expression in the Y axis; B: ENCORI database was used to analyze the PTEN expression in THCA tumor tissues; C–D: RT-qPCR and West- ern blot were employed to confirm mRNA and protein levels of PTEN in normal thyroid follicular epithelial cells Nthy-ori 3–1 and THCA cells B-CPAP, FTC-133, and TPC-1, respectively. Data were expressed as mean ± SD, and cellular experiments were repeated three times indepen- dently. One-way analysis of variance was utilized for comparison among multiple groups, * P < 0.05, *** P < 0.001

Journal: Discover oncology

Article Title: PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway.

doi: 10.1007/s12672-024-01596-8

Figure Lengend Snippet: Fig. 1 PTEN was downregulated in THCA cells. A: GEPIA database was used to analyze the expression of PTEN in THCA tumor tissues; Red box plots indicated the Tumor group (n = 512); Gray box plots represented the Normal group (n = 337); Log2(TPM + 1) served as the unit of gene expression in the Y axis; B: ENCORI database was used to analyze the PTEN expression in THCA tumor tissues; C–D: RT-qPCR and West- ern blot were employed to confirm mRNA and protein levels of PTEN in normal thyroid follicular epithelial cells Nthy-ori 3–1 and THCA cells B-CPAP, FTC-133, and TPC-1, respectively. Data were expressed as mean ± SD, and cellular experiments were repeated three times indepen- dently. One-way analysis of variance was utilized for comparison among multiple groups, * P < 0.05, *** P < 0.001

Article Snippet: Normal human thyroid follicular epithelial cells Nthy-ori 3–1 (CL-0817) and human THCA cell lines B-CPAP (CL-0575), FTC-133 (CL-0644) and TPC-1 (CL-0643) were provided by Procell (Wuhan, Hubei, China).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Comparison